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. 2020 May 28;17:169. doi: 10.1186/s12974-020-01850-0
Gene Primer Sequence
GAPDH Forward 5′-TGCCTCGCTTCACCACCTTCT-3′
Reverse 5′-AGGCCGGTGCTGAGTATGTC-3′
IL-1β Forward 5′-TGGATGCTCTCATCAGGACAG-3′
Reverse 5′-GAAATGCCACCTTTTGACAGTG-3′
IL-6 Forward 5′-AGTTGTGCAATGGCAATTCTGA-3′
Reverse 5′-CTCTGGCTTTGTCTTTCTTGTTATCTTT-3′
IL-4 Forward 5′-GGTCTCAACCCCCAGCTAGA-3′
Reverse 5′-GCCGATGATCTCTCTCAAGATAT-3′
IFN-γ Forward 5′-GCTTTGCAGCTCTTCCTCAT-3′
Reverse 5′-TTTGCCAGTTCCTCCAGATA-3′
CD4 Forward 5′-TCCTAGCTGTCACTCAAGGGA-3′
Reverse 5′-TCAGAGAACTTCCAGGTGAAGA-3′
IL-13 Forward 5′-CAGGTGTGCTCCATTTCATTCTAAT-3′
Reverse 5′-ATGGCTTTTGTGCATATCAGATGCT-3′

Relative mRNA levels were calculated using the 2−ΔΔCT method and normalized to GAPDH in the same sample. The ct number of GAPDH between groups in our experiments is stable and around 16~18.