Gene | Primer | Sequence |
---|---|---|
GAPDH | Forward | 5′-TGCCTCGCTTCACCACCTTCT-3′ |
Reverse | 5′-AGGCCGGTGCTGAGTATGTC-3′ | |
IL-1β | Forward | 5′-TGGATGCTCTCATCAGGACAG-3′ |
Reverse | 5′-GAAATGCCACCTTTTGACAGTG-3′ | |
IL-6 | Forward | 5′-AGTTGTGCAATGGCAATTCTGA-3′ |
Reverse | 5′-CTCTGGCTTTGTCTTTCTTGTTATCTTT-3′ | |
IL-4 | Forward | 5′-GGTCTCAACCCCCAGCTAGA-3′ |
Reverse | 5′-GCCGATGATCTCTCTCAAGATAT-3′ | |
IFN-γ | Forward | 5′-GCTTTGCAGCTCTTCCTCAT-3′ |
Reverse | 5′-TTTGCCAGTTCCTCCAGATA-3′ | |
CD4 | Forward | 5′-TCCTAGCTGTCACTCAAGGGA-3′ |
Reverse | 5′-TCAGAGAACTTCCAGGTGAAGA-3′ | |
IL-13 | Forward | 5′-CAGGTGTGCTCCATTTCATTCTAAT-3′ |
Reverse | 5′-ATGGCTTTTGTGCATATCAGATGCT-3′ |
Relative mRNA levels were calculated using the 2−ΔΔCT method and normalized to GAPDH in the same sample. The ct number of GAPDH between groups in our experiments is stable and around 16~18.