Fig. 1.

TRIM72 expression in the lung is induced by various injurious stimuli. a Western blot of TRIM72 protein in wildtype (WT) lungs received normal tidal volume (NV) or injurious ventilation (IV) for 3 h, intra-tracheal (i.t.) injection of PBS, 0.1 N hydrochloride acid (HCl) or 2 U/kg bleomycin (bleo). Tissues were harvested from mice 24 h after HCl i.t. or 14 d after bleo i.t.. The molecular weight (kDa) of proteins was labeled on all Western images; b quantification of TRIM72 protein or Trim72 mRNA in IV vs. NV or HCl vs PBS lungs. Band intensity is normalized to β-actin and shown in mean ± SEM, n = 3 for NV and IV, n = 3 for PBS, n = 4 for HCl, *P < 0.05, **P < 0.01 compared to NV or PBS based on two-tailed student t-test