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. 2020 May 29;21:132. doi: 10.1186/s12931-020-01384-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — TRIM72 protein expression and distribution in the IPF lung. a Western blot and quantification of TRIM72 protein in histologically normal para-tumor (control, CTRL) human lung specimens and pathologically confirmed idiopathic pulmonary fibrosis (IPF) lung specimens. n = 6 for CTRL or IPF groups; b immunostaining of TRIM72 and HT2–280 on CTRL and IPF human lung sections. HT2–280 is a membrane-bound marker for human type II alveolar epithelial cells (ATII). Competitive immunostaining using 10 μg/ml recombinant human TRIM72 protein (rhT72) was included as a control for staining specificity of the anti-human TRIM72 antibody. White arrows = TRIM72 positive ATII cells; asterisks = cellular location of TRIM72. Scale bar = 20 μm for full images, = 5 μm for high magnification images