Fig. 7.

TRIM72 maintains alveolar epithelial integrity in stressed lungs. a Immunostaining of T1α to indicate alveolar epithelial integrity in PBS- and bleo-treated B6 WT, T72KO, and T72OE (Dox injected) lungs. Scale bar = 100 μm; b H&E staining of bleo-treated WT, T72KO, and T72OE lungs. Scale bar = 100 μm. The lungs from 2 to 3-month-old B6 WT and 5–6-month-old Dox-injected 129/B6 WT mice showed no difference in immunostaining of T1α or H&E staining; c. validation of experimental models. a Western blot shows good efficiency of Dox-induced TRIM72OE transgene induction and lack of TRIM72 expression in the TRIM72 knockout (KO) lungs; d injury scores based on T1α-staining indicated epithelial disruption. Mann Whitney U test was used since the injury scores are non-parametric data. The results indicated statistically significant differences; e relative mRNA expression levels of Cdh1 (E-cadherin), sftpc, Hopx, and Aqp5 in bleo-treated lungs, n = 4 for PBS groups and n = 6 for bleo groups; *P < 0.05 or **P < 0.01 compared to WT groups based on two-sided student t-tests