Fig. 9.

TRIM72 protects bleomycin (bleo)-induced lung injury and fibrosis. a Masson’s trichrome staining in bleo-treated B6 WT, T72KO, Dox-injected WT, and T72OE lungs. Based on the darkness of the blue stain from Trichrome staining, Dox-injected 5–6-month-old WT control had more collagen deposition than 2–3-month-old B6 WT control receiving PBS i.t. Scale bar = 100 μm; b hydroxyproline contents (normalized to WT-PBS controls), and relative mRNA expression of α-SMA, Col1a1 (collagen 1 a1) and Fn (fibronectin) in bleo-treated B6 WT, T72KO, Dox-injected WT, and T72OE lungs. n = 4 for PBS groups and n = 6 for bleo groups, *P < 0.05, or **P < 0.01 based on one-way ANOVA with post hoc analysis