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. 2020 May 29;21:132. doi: 10.1186/s12931-020-01384-2

Table 1.

Nucleotide sequences of primers used for real-time PCR

Gene Direction Primer sequence GenBank
Accession #
Trim72

Forward

Reverse

5′- CCGGCAAGGCTAGATATCCA − 3′

5′- CTTCTGGTCTGAGCACTCCA − 3′

NM_001079932.3
a-SMA

Forward

Reverse

5′- GCTGGTGATGATGCTCCCA − 3′

5′- GCCCATTCCAACCATTACTCC − 3’

XM_006526606.2
Fn1

Forward

Reverse

5’- GTGTAGCACAACTTCCAATTACGAA − 3′

5′- GGAATTTCCGCCTCGAGTCT − 3’

XM_006495697.3

Col1a1

Cdh1

Sftpc

Forward

Reverse

5’- CCAAGAAGACATCCCTGAAGTCA − 3′

5′- TGCACGTCATCGCACACA − 3′

NM_007742.4

Forward

Reverse

Forward

Reverse

5′- CAGCCTTCTTTTCGGAAGACT − 3′

5′- GGTAGACAGCTCCCTATGACTG − 3′

NM_009864.3

5′- GAAGATGGCTCCAGAGAGCATC − 3′

5′- GGACTCGGAACCAGTATCATGC − 3’

NM_011359.2
Hopx

Forward

Reverse

5’- CAACAAGGTCAACAAGCACCC − 3′

5′- GGCGCTGCTTAAACCATTTCT − 3’

NM_175606.3
Aqp5

Forward

Reverse

5’- ATGAATCGGTTCAGCCCCTC − 3′

5′- TCGATGGTCTTCTTCCGCTC − 3’

NM_009701.4
Gadph

Forward

Reverse

5’- CGACTTCAACAGCAACTCCCACTCTTCC − 3′

5′- TGGGTGGTCCAGGGTTTCTTACTCCTT − 3’

XM-017321385.1

Trim72, tripartite motif-containing protein 72; a-SMA, alpha-smooth muscle actin; Fn1, fibronectin-1; Col1a1, collagen, type 1, alpha 1; Cdh1, E-Cadherin; Sftpc, surfactant protein C; Gapdh, glyceraldehyde 3-phosphate dehydrogenase