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. Author manuscript; available in PMC: 2020 May 29.
Published in final edited form as: Nature. 2019 Aug 21;573(7773):230–234. doi: 10.1038/s41586-019-1499-2

Fig 3. Piezo1 channels become flatter in large vesicles.

Fig 3.

(a) Cryo-EM image of a vesicle with a Piezo1 channel in side-view. Membrane curvatures for the region centered on Piezo1 and on the opposite pole (1166 particles). (b) Comparison of the average membrane densities at the opposite pole (top) and at Piezo1 (bottom). Vesicles with 13 nm (n=19), 19 nm (n=25) and 31 nm (n=19) curvature radius (opposite pole) are shown. (c) Circles defining the radius of curvature for outer (red) and inner (blue) membrane leaflets at the opposite pole (top) and at Piezo1 (bottom). (d) The midplane radius of curvature for Piezo1 is graphed against the midplane radius of curvature at the opposite pole (circles and dashed curve). The straight dotted line shows the relationship for spherical vesicles. Data are mean ±95% confidence intervals of the fitted radii (n ≥ 15).