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. Author manuscript; available in PMC: 2020 May 29.
Published in final edited form as: Nature. 2019 Aug 21;573(7773):230–234. doi: 10.1038/s41586-019-1499-2

Extended Data Fig. 2. Image processing procedure to determine the radius of curvature of side-view Piezo1 channels and the intrinsic radius of curvature of the vesicles in which they are embedded.

Extended Data Fig. 2.

(a) The input 2D averaged image of one side of the vesicle (here Piezo1 occupied side). (b) Edge detection output using the Canny method. (c) Edge detection output overlaid with the selection polygon. (d) Edges in the selected polygon region annotated with numbers in different colors for easier identification. (e) and (f) Measured (blue circle) and fitted (red dotted line) circles of the edges corresponding to the outer (e) and inner (f) boundaries of the vesicle membrane. Center coordinates, radius and its 95% confidence interval are shown. The unit of all values is in pixels. (g) Fitted circles (red dashed lines) overlaid onto the edge detection output. Radii with the confidence interval of outer and inner boundaries are shown in units of nm. (h) Fitted circles (red dashed lines) overlaid onto the input 2D averaged image.