Phylogenetic time tree showing the separation between the historical, EU, and LA clades. Gray brackets indicate historical clades; blue brackets, EU clades; orange brackets, LA clades. Tips in the tree are aligned to the year of isolation of the strains. Nodes are dated in the x-axis as estimated by BEAST (43). Arrow indicates the node and year of separation between EU and LA clades around 1987. *Sample from a turkey imported from Israel. †Undefined sample material. ‡Plasmids are indicated when resistance genes and plasmid replicons were found in the same contig. §IncI1/ST12 plasmids encoded CMY-2 only. EU, European; LA, Latin American. Scale bar indicates number of years.