Table 7.2.

Host factors identified using functional genomics screening technologies.

Pathogen	No. of genes being targeted in the library	Gene family target	Major pathways identified	Host factors identified	Reference
Bacterial
Brucella spp.	240	ER-associated proteins	Inositol metabolism, eukaryotic unfolded protein response	IRE1	[70]
Coxiella burnetii	21,121	Whole genome	Retromer complex	VPS26, VPS29, VPS35	[71]
Francisella tularensis	∼47,400 transcripts	Whole genome	Multiple pathways	TNFRSF9, SERPINI1	[74]
	∼21,300	Drosophila Whole genome	Lysosomal fusion and phagosomal escape	PI4KCA, USP22, CDC27	[13]
Yersinia pestis	17,370	Whole genome	Endosome recycling	RAB4A	[14]
Viral
Arenaviridae JUNV	9000	Druggable genome	Viral entry, ubiquitin ligase	VGCC, ARFRP1, CLDN2, CSDC2, KSR2, LTBP2, SSU72, TRIM2	[75]
Filoviridae EBOV	∼800,000 insertions in introns	Whole genome	Fusion of endosomes and lysosomes/biogenesis of endosomes, lysosomes, luminal cargo to the endocytic pathway, cholesterol transporter	NPC1, HOPS complex, CTSB, PIKEYVE, GNPTAB	[76]
	19,050	Whole genome	Sugar transporter involves in lysosome function, Zinc transporter protein, HOPS complex	GNPTAB, CTSB, CTSL, NPC1, SPNS1, SLC30A1, HOPS complex	[77]
	N/A	N/A	HOPS complex, endosome maturation, lysosomal protein	NPC1, CTSB, BRI3, FIG4, PIKFYVE, VPS16/39/41, RAB39B	[78]
	21,566	Whole genome	De novo pyrimidine synthesis pathway	CAD, NXF1, DDX39B	[79]
	720	Kinome	Proteins in PI3K and calcium/calmodulin kinase related network	PI3K, CAMK2	[80]
Filoviridae MARV	21,585	Whole genome	biosynthesis of heparan sulfate, MARV entry, HOPS complex	CTSL, EXT1, NPC1, HPS complex	[81]
Paramyxoviridae Henipavirus	18,120	Whole genome	Ribosomal biogenesis, nuclear export/import, transcriptional regulation, rDNA transcription, pre-rRNA cleavage, subunit assembly, chemical modification of pre-rRNA	RPL, RPS, ESF1, XOP1, KPNA3, BTF3, SP7, POLR3E, DDX10, IMP4, GTPBP4, RPL13A, FBL	[82]
Poxviridae Vaccinia virus	6719	Druggable genome	Translation of mRNA, ceullar transcriptional processes, DNA repair pathways, AMPK complex, GTP binding proteins	153 pro- and 149 antiviral host factors	[83]
	∼440	Drosophila kinome	AMPK pathway	PRKAA2, PRKAG2, PRKAB1, PIKFYVE, PIK3C2A, STAM, PTPN23, MYLK	[84]
	21,566	Whole genome	Translational, ubiquitin-proteasome, and ER-to-Golgi transport function, RNA polymerase II	NUP62	[85]
Togaviridae VEEV	140	Genes involved in trafficking	Actin rearrangements, trafficking in trans-Golgi network	Rac1, PIP5K1-a, Arp2/3 complex	[86]