Figure 2.

CH decreases pulmonary arterial smooth muscle membrane cholesterol. (A) Representative images of filipin fluorescence (arbitrary units [AU]) as a marker of membrane cholesterol content in pulmonary arteries from control and CH rats after treatment with vehicle, methyl-β-cyclodextrin (MβCD) (10 mM), or supplemental cholesterol. Scale bar: 50 μm. (B) Mean filipin fluorescence in control and CH arteries under vehicle, MβCD, and cholesterol repletion conditions. Values are means ± SE of n = 5–7 animals/group. *P < 0.05 versus respective control. ^#P < 0.05 versus respective vehicle.