Different effects of ORMDL3 knockdown on replication of various RV strains. HeLa-E8 cells were transfected with ORMDL3 or control siRNA and infected with three strains of RV-A (1, 16, and 35), RV-B52, and three strains of RV-C (2, 15, and 40). Forty-eight hours later, RNA was harvested and quantitated for RV by qPCR. Levels of RV in the ORMDL3 knockdown cells were normalized to control siRNA-transfected cells (control SiRNA set = 1; N = 4 experiments). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.005, and ****P ≤ 0.001.