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. Author manuscript; available in PMC: 2021 Mar 3.
Published in final edited form as: Cell Metab. 2020 Feb 21;31(3):605–622.e10. doi: 10.1016/j.cmet.2020.01.013

Figure 4. Targeting Glutaminase 1 (GLS1) in vitro and in vivo restores very-low-density lipoproteins (VLDL) triglyceride export after choline and methionine deprivation.

Figure 4.

A. Representative BODIPY staining micrographs and respective quantification in mouse isolated hepatocytes treated with control media (Ctrl) or methionine- and choline -deficient media (MCD) for 24 h after overnight treatment with siRNA against Gls1 (siGls1) or unrelated control (siCtrl). In some experimental conditions lomitapide was added at 600 nM for 24 hours. Scale bar corresponds to 100 μm. At least triplicates were used for each experimental condition. Data is shown as average ± SEM and Student’s t-test was used to compare between groups. *p<0.05 and **p<0.01 versus Ctrl + siCtrl and ##p<0.01 versus MCD + siCtrl are shown. B. Liver phosphatidylcholine (Ptd-Cho), phosphatidylethanolamine (Ptd-Et) and phosphatidylserine (Ptd-Ser) hepatic levels and C. Serum very-low-density lipoprotein (VLDL) phospholipids and lipid content in mice fed either a standard chow (SC) diet or a diet deficient in choline with 0.1% methionine (0.1% MCDD) for four weeks. From weeks two to four of diet, two different experimental groups were treated either with siCtrl or siGls1. Animals were administered poloxamer 407 (P407) and serum VLDL isolated and analyzed at six hours after P407 administration. At least n=5 were used for each experimental group. Data is shown as average ± SEM and one-way ANOVA followed by Bonferroni post-test was used to compare between multiple groups. **p<0.01, ***p<0.001 and ****p<0.0001 versus SC diet and #p<0.05 and ##p<0.01 versus 0.1%MCDD + siCtrl are shown (See also Supplemental Figure 7).