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. 2018 Mar 1;1:41–99. doi: 10.3114/fuse.2018.01.04

Table 2.

List of primers used for amplification and sequencing in this study.

Name1 Sequence (5’ – 3’) Orientation Reference
ITS
ITS4 TCC TCC GCT TAT TGA TAT GC Reverse White et al. (1990)
ITS5 GGA AGT AAA AGT CGT AAC AAG G Forward White et al. (1990)
LSU
LR0R GTA CCC GCT GAA CTT AAG C Forward Rehner & Samuels (1994)
LR5 TCC TGA GGG AAA CTT CG Reverse Vilgalys & Hester (1990)
rpb2
RPB2-5F2 GGG GWG AYC AGA AGA AGG C Forward Sung et al. (2007)
RPB2-7cR CCC ATR GCT TGY TTR CCC AT Reverse Liu et al. (1999)
tef1
EF-2 GGA RGT ACC AGT SAT CAT GTT Reverse O’Donnell et al. (1998)
EF1-728F CAT CGA GAA GTT CGA GAA GG Forward Carbone & Kohn (1999)
tub2
Bt2a GGT AAC CAA ATC GGT GCT GCT TTC Forward Glass & Donaldson (1995)
Bt2b ACC CTC AGT GTA GTG ACC CTT GGC Reverse Glass & Donaldson (1995)

1ITS: internal transcribed spacers and intervening 5.8S nrDNA; LSU: large subunit (28S) of the nrRNA gene operon; rpb2: partial DNA-directed RNA polymerase II second largest subunit gene; tef1: partial translation elongation factor 1-alpha gene; tub2: partial beta-tubulin gene.