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. 2020 May 25;13:4617–4625. doi: 10.2147/OTT.S234667

Figure 3.

Figure 3

SASH1 knockdown induces the cell viability and invasion of HSSCC cells. HSSCC cell lines transfecting with SASH1 siRNA or NC siRNA for 48 h were constructed. The expression levels of SASH1 mRNA (A) and protein (B) were determined using RT-PCR and Western blotting, respectively. (C) Cell viability was measured using the CCK-8 assay. (D) Colony formation was evaluated using the colony formation assay. (E) Cell apoptosis was measured using the caspase-3 activity test. (F) Cell invasion was assessed by the transwell invasion assay. Compared with NC siRNA group. *P<0.05.