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. 2020 May 21;5(10):e136653. doi: 10.1172/jci.insight.136653

Figure 1. Vaccination with HA-ferritin nanoparticles elicits enhanced antibody titers and protective immunity.

Figure 1

C57BL/6 mice (n = 5 mice per group) were vaccinated intramuscularly with high or low doses of HA-ferritin (5 or 0.5 μg) or a molar equivalent of soluble HA (3.8 or 0.38 μg). Control groups received ferritin alone (1.2 μg) or PBS. All vaccines except PBS were adjuvanted with AddaVax. (A) HA-specific serum IgG titers were measured by ELISA 14 days after vaccination. Data are representative of 1 of 2 independent experiments. The dashed line indicates detection cutoff (1:100 dilution). (B) HA-specific serum IgG titers 14 days after final vaccination in mice vaccinated 3 times at 14-day intervals with 100 μg DNA encoding HA-ferritin, soluble HA, or ferritin. (C) Body weight and survival of mice immunized once 14 days before intranasal challenge with PR8 or CA09 influenza strains. The dashed line indicates 20% weight loss. Data represent mean ± SD. *P < 0.05, and **P < 0.01, determined by a Mann-Whitney U test.