Skip to main content
. 2020 May 21;5(10):e136653. doi: 10.1172/jci.insight.136653

Figure 2. Augmented HA-specific GC responses in the draining LN following HA-ferritin vaccination.

Figure 2

(A) C57BL/6 (n = 5 mice per group) mice were immunized with HA-ferritin (5 or 0.5 μg) or a molar equivalent of soluble HA (3.8 or 0.38 μg) or 1.2 μg ferritin alone, adjuvanted with AddaVax. After 14 days, draining inguinal LNs were sectioned and stained for GCs (GL7 shown in green and B220 shown in magenta). Images are representative of each treatment group. (B) Mice were vaccinated as described for A except for AddaVax-alone group n = 2. The proportion of IgD–B220+ cells expressing GL7 in draining iliac (left) and inguinal (right) LNs was quantified by flow cytometry at 7, 14, 28, or 56 days after vaccination. (C) The absolute count of GC B cells (B220+IgD–GL7+) in draining iliac (left) and inguinal (right) LNs binding HA at 56 days after vaccination was measured using a probe of biotinylated PR8 HA labeled with streptavidin-PE. Data represent mean ± SD and are representative of 1 of 2 independent experiments. *P < 0.05, and **P < 0.01, determined by a Mann-Whitney U test.