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. 2020 May 21;5(10):e136653. doi: 10.1172/jci.insight.136653

Figure 3. Increased mutation in immunoglobulin genes of memory B cells following HA-ferritin vaccination.

Figure 3

C57BL/6 (n = 6 mice per group) mice were immunized with HA-ferritin (5 μg) or a molar equivalent of soluble HA (3.8 μg), both adjuvanted with AddaVax. (A) HA-specific GC B cells in draining LNs at days 14 and 28 after vaccination or (B) memory B cells in the spleen and blood at day 71 after vaccination were single cell sorted by FACS. Immunoglobulin heavy chain variable domain genes were amplified and sequenced. The mutation rate from germline murine variable domain sequences was determined. Data represent mean ± SD of 10–270 cells. *P < 0.05, determined by a Mann-Whitney U test.