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. 2020 May 29;15(5):e0233795. doi: 10.1371/journal.pone.0233795

Fig 4. Obesity induces minor alterations to the ccRCC immunogenetic profile.

Fig 4

(A) Volcano plot depicting immune-associated gene expression changes in ccRCC tissues from subjects with category II/III obesity (n = 12) versus non-obese (n = 12) subjects. Dotted line indicates p = 0.05. (B) Individual significantly differentially expressed genes and associated log2 fold changes from panel A. (C) nanoString-generated gene expression-derived cell type scores. Flow cytometric analysis of (D,E) tumor-infiltrating lymphocyte (TIL), (F) tumor-infiltrating myeloid (TIM) and tumor-associated macrophage (TAM) populations from ccRCC tissues of non-obese (n = 7–8) ccRCC subjects or ccRCC subjects with obesity (n = 6–7). (C-E) Data are presented as mean expression, boxes defining 25th to 75th percentiles with line at median and whiskers extending to minimum and maximum points, or means ± SEM with individual values for each subject shown. Statistical analyses were performed using parametric two-tailed unpaired student’s t-tests or non-parametric Mann-Whitney U tests. ns = not statistically significant, NOB = Non-obese BMI<30, OB = Obese BMI≥30.