Fig 1. Effect of histones on inducing inflammatory markers in human whole blood over 6 and 24 hours.

Whole blood was stimulated with increasing concentrations of histones over 6 and 24 hours. Released C3a, IL-6 and IL-8 were measured in plasma and tissue factor was measured from the cellular component. For each timepoint all concentrations were p < 0.001 relative to the background (0 μg/ml) reading by Dunnett’s multiple comparison. Error bars = standard deviation of the average from 6 donors.