Fig 3. Ability of selectively desulfated heparin to attenuate histone induced inflammatory responses in whole blood.

Histones, 50μg/ml, and differently desulfated heparins, 200 μg/ml, were added to whole blood and incubated for 6 hours. Released C3a, IL-6 and IL-8 were measured in plasma and tissue factor was measured from the cellular component. Analysis of data was carried out using Dunnett’s multiple comparison with no heparin (histones only) as the control sample, * = p < 0.05, ** = p < 0.01, *** p <0.001. Error bars = standard deviation of the average from 6 donors.