Figure 2. PRKAA1 and PRKAA2 deficiency disrupts time to first weaning with no impact on ovarian structure and function.

(A) Overall time to weaning of pups is dramatically increased in primiparous Prkaa1/2^d/d females (p=0.014). (B) Pups born to Prkaa1/2^d/d dams weighed significantly less at the time of weaning than pups born out of Prkaa1/2^fl/fl females. (C) A histological analysis demonstrated no difference in ovarian architecture or corpora lutea formation between Prkaa1/2^fl/fl and Prkaa1/2^d/d mice during early pregnancy (DOP5) or pseudopregnancy (DOPP5) (n=4–6). (D) Serum P₄ measured on DOP5 was not different between Prkaa1/2^fl/fl (n=6) and Prkaa1/2^d/d (n=4) animals demonstrating that post-ovulatory steroidogenesis is not dependent on AMPK activity. Values are expressed as mean ± SEM, *P≤0.05.