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. 2020 May 29;15(5):e0233322. doi: 10.1371/journal.pone.0233322

Table 2. Quantitative PCR primer sequences for targeted genes: PCR product sizes, average PCR efficiencies, and the mean Cycle Threshold (CT) values of gonadal cDNA samples from Atlantic salmon (n = 24).

Targeted gene GenBank accession # Forward Primer Revers Primer Product size (bp) PCR efficiency (%) Mean CT
star1 XM_014171084 GATGGGAGATTGGAACCCTAAC ACAGCGAACACTAACGAAGTCC 139 95.6 28.29
hsd3b1 XM_014174054 GAGGGGGACATTAGTGATAGTGAG GCTGGGTTCCTTTGACGTTG 142 112.8 29.75
hsd11b1 XM_014176669 CCCTCGTCTCACCCTACTACA TTATTCACCAAGCCCCACAGA 114 95.6 27.71
cyp11a (p450scc) 1 DQ361039 CTCAAGAATGGGGAGGACTG ACTTCATCCAACAGAGGAACAAAG 101 92.8 28.21
cyp11b (p450c11) 1 XM_014149090 AAAGAGGGAGGAGAGGAGGAGAG GGAGGGAGAGGTGGAGAAGAG 135 109.0 29.27
cyp17a (p450c17) 1 XM_014154002 GGCTACAGGTCTTCCCCAAC AACTTGTGTTCCTCGTATTTCTTC 97 103.3 28.79
amh1 NM_001123585 CAAAAACACCAGAGACAGGACAA TATCCGTTGAGAAAAGCACCA 111 96.2 25.81
acsl42 NM001173689 AGCACCTGAACAATGACTTCT TCCTTCTTACGGTCCACTATCT 115 94.8 27.60
acot22 GEGY01113449 GGACTTTCTCCGCACCAGAA GGGAGAGAGGCACAGGTCTA 126 86.1 26.81
lpl3 XM_014216226 GCCCGACCTTTGAGTTTGC ACGTCCACAAAGAGAGCATCGT 67 107.6 30.51
lipe (hsl)2 NM_001173663 GCCAAGAACCCGTTTGTGTC TGGCCCATATTCCGCAGTTT 143 110.5 26.92
cd363 AY606034 TTTCCTGCTGCGCACCTT GGTGCGGGTCATGAAGATTT 70 94.8 25.10
fatp3 CA373015 AGGAGAGAACGTCTCCACCA CGCATCACAGTCAAATGTCC 157 96.6 30.80
fabp113 DR695475 CCGCCGACGACAGAAAAA TTTTGCACAAGGTTGCCATTT 61 95.9 25.90
fads1 (Δ5 desaturase)4 AF478472 GAGAGCTGGCACCGACAGAG GAGCTGCATTTTTCCCATGG 51 89.1 30.77
fads2 (Δ6 desaturase)4 AY458652 AGAGCGTAGCTGACACAGCG TCCTCGGTTCTCTCTGCTCC 51 85.8 29.48
elovl24 TC91192 CGGGTACAAAATGTGCTGGT TCTGTTTGCCGATAGCCATT 123 104.6 29.50
elovl5a4 AY170327 GATGAGCGGGTACAGGGATG CGGCTGTCTGTGTCTCATGT 117 92.4 27.38
elovl5b4 DW546112 ACAAAAAGCCATGTTTATCTGAAAGA AAGTGGGTCTCTGGGGCTGTG 141 99.0 30.44
fasn (fas)2 XM014179803 CACCTTCGGTCATCTTCTGTT CAGGATGGAGGAGATTGTTGTAG 123 96.6 27.09
chpt15 GBRB01045139 GTACGCCTTCCCCATCTTGG TGAGGCCGATGTGCATACCT 149 110.4 28.80
cept15 NM_001139869 GCAGGCCAGACGAACCAATA TGAACATGCCAGCGAAACAGC 161 98.4 28.78
apoa15 NM_001123663 CTGGTCCTCGCACTAACCAT TGGACCTCTGTGCAGTCAAC 142 103.5 27.57
apob6 X81856 TTGCAGAGACCTTTAAGTTCATTCA TGTGCAGTGGTTGCCTTGAC 121 103.5 28.98
18s1 AJ427629 CTCAACACGGGAACCCTCAC AGACAAATCGCTCCACCAAC 118 92.4 10.57

1 Maugars and Schmitz [16]

2 New assay designed for the present study

3 Torstensen et al [47]

4 Thomassen et al [48]

5 Carmona-Antonanzas et al [37]

6 Gu et al [49].