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. 2020 Apr 18;12(5):674–683. doi: 10.1093/gbe/evaa079

Fig. 3.

Fig. 3.

—Stalk and spore gene expression in Pvio stlB−. (A) EcmAB1 expression pattern. Pvio wild-type and stlB− cells, transformed with the Pv_ecmAB1-lacZ vector, were incubated on nitrocellulose filters supported by NN agar until they had reached the tipped mound (a) and early (b) and late (c) primary sorogen stage. Structures were then fixed and stained with X-gal. Bars: 0.2 mm. (B) Stalk and spore transcript levels. Pvio wild-type and stlB− cells were incubated on NN agar at 4 °C overnight and then at 22 °C for 3, 6, 9, and 12 h, when they had reached the indicated stages. Total RNA was isolated and levels of ecmAB1 stalk-specific transcripts, g1612 (pre)spore-specific transcripts, and rpb5 constitutively expressed transcripts were determined by RT-qPCR. Data for ecmAB1 and g1612 were normalized to rpb5 transcript levels and expressed relative to transcript levels at 12 h for wild-type cells. Means and SD of three experiments, assayed with three technical replicates each, are presented. Asterisks indicate significant differences between wild-type and stlB− transcript levels (t-test, P<0.05).