Fig. 4.

—Maximum likelihood phylogenies for the cifA and cifB genes, and their protein structures. Phylogenetic gene trees were constructed from codon alignments of the wCer2 cif genes plus reference sequences of the Types I–V orthologs obtained from GenBank. The archetypal cif genes are named according to the current GenBank annotation for consistency with other reference sequences: Here, wMel_RS02835 = WD0631 = cifA; wMel_RS06940 = WD0632 = cifB. The general domain structure of each cif gene type is shown, including the CIFA domains catalase (red); DUF3243 (light green); STE (light blue); Ulp1 deubiquitinase module in CIFB (dark blue); and the PDDEXK nuclease domains (yellow). The scale bars represent the number of substitutions per site.