Table 1.
Genome Reads Obtained from Five Different Genomic Host Libraries and Their Mapping Success to wCer2 and Mitochondrial Host Genomes
| Host Species | Line (Previous Name) | Donor (Host Plant) | Source (Isolation Strategy) | Wolbachia Strain | No. of Paired Reads after QC | No. Paired Reads Mapped to wCer2 | Percentage Mapped to wCer2 | Percentage Mapped to mtDNA |
|---|---|---|---|---|---|---|---|---|
| Drosophila simulans | DsimRC50 (RC50) | RcerAV (honeysuckle) | Embryos (purified Wolbachia cells) | wCer2 | 39,480,884 | 31,178,350 | 78.99% | 11.48% |
| 2,200,000 (sampled) | 1,736,722 | 78.94% | 11.47% | |||||
| DsimRC45 (RC45) | RcerAV (honeysuckle) | Embryos (purified Wolbachia cells) | wCer2 | 191,317,107 | 145,719,874 | 76.17% | 14.09% | |
| 2,200,000 (sampled) | 1,682,758 | 76.49% | 14.09% | |||||
| Drosophila melanogaster | DmelRC13A | DsimRC50 | Embryos (purified Wolbachia cells) | wCer2 | 110,991,840 | 100,278 | 0.09% | 91.44% |
| Ceratitis capitata | Ccap88.6 (WolMed 88.6) | RcerAS (cherries) | Single adult (abdomen) | wCer2 | 117,173,724 | 1,667,076 | 1.42% | 16.57% |
| Rhagoletis cerasi | RcerAS; Stillfried, Austria | Not applicable | Single pupa (whole individual) | wCer1; wCer2; wCer5 | 131,385,710 | 3,341,178 | 2.54% | 43.15% |
| 66,000,000 (sampled) | 1,681,128 | 2.55% | 43.16% |
Note.—Some libraries were subsampled to normalize the number of wCer2 reads for variant calling, based on the lowest number of wCer2 in Ccap88.6 (excluding the insufficient coverage of wCer2 in DmelRC13A). The percentage of mapping success is calculated accordingly.