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. 2020 May 29;15(5):e0233750. doi: 10.1371/journal.pone.0233750

Fig 2. Dysfunction of BRCA1 affects glycolysis related gene PFKP expression.

Fig 2

(A-D) The expression level of glycolysis associated genes in BRCA1-deficient mouse embryonic fibroblasts MEF BRCA1△/△ cells and wild-type MEF BRCA1+/+ cells. (E) Real time PCR confirmed the mRNA level of PFKP in MEF BRCA1+/+ cells and MEF BRCA1△/△ cells. (F) Western blot verified the protein level in MEF BRCA1+/+ cells and MEF BRCA1△/△ cells. (G) Western blot and real time PCR detect the expression of PFKP in BRCA1 overexpressed HCC1937 cells and control counterparts. (H) Western blot and real time PCR examined the expression of PFKP in BRCA1 knocked down MCF-7 cells and control groups. All experiments were biological replications. ***p < 0.001. **p < 0.01.*p < 0.05.