Table 1. Quantification of the embryonic heart phenotype of CAP mutants, UAS-CAP RNAi, CAP mutant rescue and of CAP double heterozygous animals.
Hearts are categorized as defective if displaced or misaligned Mef2-stained CB nuclei where observed.
| Quantification of the CAP phenotype | |||
|---|---|---|---|
| Genotype | Heart defects | Mef expressing cardioblasts | Doc expressing cardioblasts |
| w1118 | 0% (n = 52) | 103.5 ± 1.2 (n = 22) | 28,2 ± 0.7 (n = 22) |
| CAP42b | 64% (n = 115) | 107.5 ± 1.2 (n = 27) | 28,4 ± 0.9 (n = 27) |
| CAP42b/Df(2R)BSC281 | 53% (n = 17) | 106.3 ± 1.9 (n = 10) | 28,4 ± 1.2 (n = 10) |
| CAP49e | 63% (n = 75) | 106.2 ± 2.6 (n = 29) | 28,6 ± 0.9 (n = 29) |
| CAP49e/Df(2R)BSC281 | 55% (n = 20) | 105.8 ± 2.9 (n = 8) | 28,9 ± 1.5 (n = 8) |
| Df(2R)BSC281 | 72% (n = 18) | n.d. | n.d. |
| UAS-CAP RNAi | 0% (n = 15) | 104.0 ± 0 (n = 15) | 28,2 ± 0.4 (n = 15) |
| UAS-CAP RNAi; tinC+D-GAL4 | 41% (n = 12) | 104.0 ± 0 (n = 12) | 28,0 ± 0.3 (n = 12) |
| UAS-CAP RNAi; twi+how-GAL4 | 64% (n = 14) | 104.0 ± 1.0 (n = 14) | 29,0 ± 1.0 (n = 14) |
| CAP49e;UAS-CAP-C/tinCΔ4Gal4 | 26% (n = 19) | 104.3 ± 1.2 (n = 8) | 28,3 ± 0.7 (n = 8) |
| CAP49e; rhea1 | 47% (n = 17) | 105.7 ± 2.0 (n = 6) | 28,2 ± 0.4 (n = 6) |
| CAP49e/scb2 | 48% (n = 29) | 104.2 ± 1.1 (n = 11) | 28,1 ± 0.3 (n = 11) |