Fig 1. Quantification of total biomass of biofilms.

M. haemolytica (D153) and S. aureus Newbould 305 (NB305) were grown in BHI broth and colorless RPMI 1640 in 24-well plates at 37°C for 48 h (NB305) to 72 h (D153). The effects of growth media (A) and bacteria (B) on the amount of biofilm productions were compared with each other. Biofilms were stained with crystal violet and the absorbance of released crystal violet in glacial acetic acid was measured at 630 nm (OD₆₃₀). Bars indicate mean and standard deviation of total biomass of biofilms from three independent experiments performed in six replicate wells are shown. Differences between biomass of biofilms were determined by Student’s t-test. * P < 0.0001.