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. 2020 May 29;15(5):e0233973. doi: 10.1371/journal.pone.0233973

Fig 2. Confocal laser scanning microscopy of biofilms.

Fig 2

M. haemolytica (D153; 20× objective lens: A and B; 60× objective lens: E and F) and S. aureus Newbould 305 (NB305; 20× objective lens: C and D; 60× objective lens: G and H) were grown in BHI broth (A, C, E, and G) and colorless RPMI 1640 (B, D, F, and H) in 96-well plates at 37°C for 48 h (NB305) to 72 h (D153). Biofilms grown on polystyrene plastic surfaces (A-D) and polymer coverslips (E-H) were stained with Filmtracer™ LIVE/DEAD™ Biofilm Viability stains (SYTO 9 and propidium iodide) and multiple z-stacks were collected per each sample and three-dimensional images were produced using Nikon software. One representative biofilm image out of two independent experiments performed in triplicate wells are shown.