Table 1. Flow cytometry results of live and dead bacteria in the biofilms.

	BHI		RPMI 1640
	Live	Dead	Live	Dead
M. haemolytica (D153)	71.9 ± 3.3	27.2 ± 3.3	80.7 ± 1.5	19.0 ± 1.5
S. aureus (NB305)	65.9 ± 1.8	34.1 ± 1.8	96.7 ± 1.2	3.3 ± 1.2

M. haemolytica (D153) and S. aureus Newbould 305 (NB305) were grown in BHI broth and RPMI 1640 in 96-well plates at 37°C for 48 h (NB305) to 72 h (D153). Bacteria in the biofilms were stained with Filmtracer™ LIVE/DEAD™ Biofilm Viability stains and the viability was determined by flow cytometry analysis. Mean percentages of live and dead bacteria with corresponding SD are shown.