Table 2. EQI decreases malonyl-CoA levels in S. aureus.
| Fold-change (EQI vs. DMSO) | p-value | ||
|---|---|---|---|
| Malonyl-CoA | 0.20 | <0.01 | |
| AMP | 0.27 | 0.01 | |
| NAD+ | 0.48 | 0.16 | |
| ADP | 0.71 | 0.01 | |
| ATP | 0.79 | 0.14 | |
| GDP | 0.87 | 0.50 | |
| NADP+ | 0.91 | 0.62 | |
| Adenine | 0.94 | 0.70 | |
| Pyruvate | 0.96 | 0.94 | |
| Acetyl-CoA | 1.21 | 0.26 | |
| Glycerol-3P | 1.31 | 0.20 | |
| NADH | 1.37 | 0.70 | |
| Glutamate | 1.38 | <0.01 | |
| BSSB | 1.42 | 0.21 | |
| CoASH | 1.49 | 0.38 | |
| Proline | 1.56 | <0.01 | |
| Alanine | 1.60 | <0.01 | |
| Succinate | 1.67 | <0.01 | |
| BSH | 1.70 | <0.01 | |
| Glutamine | 1.79 | <0.01 | |
| Fumarate | 1.95 | <0.01 | |
| GTP | 2.11 | 0.01 | |
| NADPH | 3.24 | 0.07 | |
| Adenosine | 4.21 | <0.01 | |
| Malate | 5.52 | 0.03 | |
| Lactate | 7.13 | 0.07 | |
S. aureus cultures were treated with EQI (4.36 μM) for 2 h in quintuplicate. Metabolites were quantified by LC-MS. Metabolites were normalized to bacterial concentrations and fold-changes were determined in comparison to DMSO. Significance of fold-change was determined using a two-tailed, unpaired t tests; DMSO vs EQI. Analytes with a p < 0.05 are indicated in red.