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. 2020 May 18;16(5):e1008579. doi: 10.1371/journal.ppat.1008579

Fig 6. Car1+ c-Kit+ β7+ CD24a+ cells are mast cell-erythrocyte progenitors.

Fig 6

(A), Car1-GFP+ c-Kit+ β7+ CD24a- cells were sort-purified, seeded into MethoCult and the number of colonies were quantified post-culture. (B), Representative colony from Car1-GFP+ c-Kit+ β7+ CD24a- seeded cultures. (C), Single colonies were isolated from Car1-GFP+ c-Kit+ β7+ CD24a- seeded cultures and the presence of erythrocytes and mast cells were determined by flow cytometric analysis. (D), The values for the gated cell populations illustrated in (C) were plotted for each colony isolated. (E), Car1-GFP+ c-Kit+ β7+ CD24a+ cells were sort-purified, seeded into MethoCult and the number of colonies were quantified post-culture. (F), Representative colony from Car1-GFP+ c-Kit+ β7+ CD24a+ seeded cultures. (G), Single colonies were isolated from Car1-GFP+ c-Kit+ β7+ CD24a+ seeded cultures and the presence of erythrocytes and mast cells were determined by flow cytometric analysis. (H), The values for the gated cell populations illustrated in (G) were plotted for each colony isolated. (A-G), Representative of at least 3 separate experiments. At least 10 single colonies were isolated per experiment.