Fig 7. Car1-GFP+ HPCs support mast cell and erythrocyte development post-Trichinella infection.

Car1-GFP+ cells were sort-purified from the bone marrow of naïve CD45.1 congenic mice and adoptively transferred (i.p.) into CD45.2 hosts on day 4 and 5 post-infection. On day 10 post-infection (A) peritoneal mast cells were identified as CD45+ c-Kit+ IgE+ and (B) the percent of CD45.1 and CD45.2 mast cells were determined in control mice and mice receiving cell transfers. (D), Erythrocytes in the peritoneal exudate identified as CD45- TER119+ and (E) the percent of erythrocytes expressing Car1-GFP were determined in control mice and mice receiving cell transfers. (C, F), The percentage of identified cells were quantified. WT and Cpa3-Cre mice were infected with T. Spiralis. Groups of Cpa3-Cre mice received transfers of Car1-GFP+ cells post-infection and (G, H) peritoneal mast cells, (I) IL-4, IL-5 and IL-13 levels of stimulated mLNs (J) and worm burdens were determined. (A,D,G), Representative plots illustrating gating strategies. (A-F) Are representative of at least 3 separate experiments. (G,H), Results are representative of 2 separate experiments comprised of 10 biological replicates total. Statistical analysis performed using a Student’s t-test.