Table 2.

Proposed reporting standards for mouse models of diabetes-associated ulceration

Elevated and sustained glycemia	Diabetes should be validated by more than one method, as per recommendations by DiaComp, and, ideally, performed multiple times. Suggested criteria: Fasting blood glucose >8.33 mmol/L (highly recommended) or otherwise random blood glucose >15 mmol/L. Validation via intraperitoneal glucose tolerance test, or euglycemic clamp on awake and conscious animals.
Presence of ischemia	May be examined using laser Doppler or similar techniques. May be artificially induced via ligation/obstruction/excision of local major artery.
Presence of neuropathy	May be examined by electrophysiology, behavioral tests and/or histology.
Location of wound on periphery	Ideally on the foot/paw.
Possible infection could be considered	May be artificially induced.

DiaComp, Diabetic Complications Consortium.