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. 2020 May 29;6(22):eaay7148. doi: 10.1126/sciadv.aay7148

Fig. 5. MSN-PEI 25K attenuates multiple organ injury in CLP-induced severe sepsis.

Fig. 5

High-grade CLP was performed on BALB/c mice, followed by treatment as described in Fig. 4. (A) Twenty-four hours after CLP, the lung, kidney, heart, liver, and spleen tissues were collected, stained with H&E, and analyzed. Scale bars, 20 μm. Multiple organ injury representations, including interalveolar septum thickened in the lung, tubular epithelial cell swelling in the kidney, inflammatory cell infiltration in the liver and heart, and nuclear debris from dying cells in the spleen, are marked with arrows. The corresponding (B) liver and (E) kidney injury scores were determined and calculated according to the established criteria. In parallel, the blood serum biochemistry parameters (C) ALT, (D) AST, (F) BUN, and (G) CRE were measured. Differences were assessed via one-way ANOVA with Tukey’s multiple comparison tests (n = 6 mice per group; *P < 0.05, **P < 0.01, and ***P < 0.001). The data are expressed as the means ± SEM.