Fig. 5. Demonstration of sorting budding yeast cells with low proliferative activity.
(A) Experimental procedure. (i) Strains of adh1Δ and YEF8045 were mixed at a ratio of 1:49, (ii) encapsulated in ~1-nl droplets, (iii) incubated for 12 hours, and (iv) reinjected and sorted with the SADA sorter. (B and C) Bright-field images of SADA-unsorted (target) and SADA-sorted (nontarget) droplets, respectively. (D) Histogram of the volume occupied by budding yeast cells within each droplet [n = 182 droplets for unsorted (target) droplets and n = 240 droplets for sorted (nontarget) droplets]. Defining the threshold for the low proliferative activity cutoff to be 1.5 × 105 μm3, the purities of the target and nontarget droplets in the corresponding channels were both found to be 93%. Scale bars, 100 μm.