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. 2020 May 4;130(6):2966–2977. doi: 10.1172/JCI133753

Figure 6. HDAC7 indirectly regulates c-Myc expression.

Figure 6

qRT-PCR (n = 6) and Western blotting (n = 4) for c-Myc expression in NRVMs treated with siRNA against Hdac7 (A and B), Ad-HDAC7 (C and D), or YKL-05-099 (1 μM) (E and F). All box plots show minimum, maximum, and median with 25th to 75th percentile range. (G) Western blotting of cytoplasmic (C) and nuclear (N) fractions of NRVMs treated with 1 μM YKL-05-099 plus 100 μM PE. TATA box–binding protein (TBP) and GAPDH were used as nuclear and cytoplasmic markers, respectively (representative Western blots, n = 3). (H) NRVMs treated with adenovirus harboring GFP-HDAC7 or GFP alone, followed by transfection of siRNA probes against c-Myc or scramble control. Immunostained for α-actinin (red) and nuclei (blue). Scale bars: 40 μm. (I) Cell area of NRVMs (n = 100). Bars denote mean ± SD. (J) qRT-PCR expression for Nppa and Nppb (n = 6). Data are shown as means ± SEM unless noted. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, by 1-way ANOVA with Tukey’s multiple comparisons test.