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. 2020 May 11;130(6):3098–3112. doi: 10.1172/JCI130546

Figure 1. Neutrophil depletion during chronic TB improves Mtb control.

Figure 1

(A) The numbers of blood neutrophils from Rhesus macaques with ATB (n = 8) or LTBI (n = 10) were measured at dpi following Mtb infection or at necropsy (N). B6 mice (n = 5–13) were aerosol infected with approximately 100 CFU Mtb HN878. (B) Neutrophil accumulation was determined by flow cytometry at dpi. B6 mice were infected with Mtb HN878 and administered IgG (n = 5–10) or 1A8 (n = 5–10, 300-μg/dose) i.p. every other day. (C) Bacterial burden in the lung and spleen was determined by plating on 21 dpi. (D) Lung homogenates were analyzed for G-CSF protein expression by ELISA on 21 dpi. (E) Pulmonary inflammation was quantified on formalin-fixed, paraffin-embedded (FFPE) lung sections from 21 dpi samples stained with H&E. B6 mice were infected with HN878 and administered IgG (n = 4–5) or 1A8 (n = 4–5, 300-μg/dose) i.p. every other day. (F) Bacterial burden in the lung and spleen was determined by plating on 106 dpi. (G) Lung homogenates were analyzed to measure levels of IL-6 and TNF-α proteins at 106 dpi using Luminex assays. (H) Pulmonary inflammation was quantified on FFPE lung sections from 106 dpi tissues stained with H&E. Figures depict 1 experiment representative of 2 or combined data from multiple experiments. Data points represent the mean ± SEM of values. (A) 2-way ANOVA; (B) Student’s t test between 0 and indicated dpi; (C–G) Student’s t test between isotype- and 1A8-treated mice. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.