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. 2020 May 11;130(6):3098–3112. doi: 10.1172/JCI130546

Figure 3. S100A8/A9 deficiency protects mice during chronic TB.

Figure 3

B6 (n = 5–10) and S100A9KO (n = 5–9) mice were aerosol infected with approximately 100 CFU Mtb HN878. (A and B) Lung and spleen bacterial burden were determined by plating at different dpi. Lung myeloid population cell counts were enumerated in B6 and S100A9KO HN878-infected mice using flow cytometry at 50, 100, 150, and 300 dpi. (C) Neutrophils and (D) other myeloid cells are shown. (E) FFPE lung sections were used to carry out immunofluorescence staining for Ly6G (green), MPO (red), and DAPI (blue) or B220 (green), CD3 (red), and DAPI (blue). (F) Total area occupied by B cell follicles was determined using the morphometric tool of the Zeiss Axioplan microscope. Original magnification, ×200. Figures depict 1 experiment representative of 2 or combined data from multiple experiments. Data points represent the mean ± SEM of values. (A–F) Student’s t test between B6 and S100A9KO at each indicated time point. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.