Skip to main content
. 2020 May 4;130(6):2953–2965. doi: 10.1172/JCI123159

Figure 4. NAD+-mediated enhancement of NER efficiency requires SIRT2 and is independent of SIRT1.

Figure 4

(A) Western blot showing the activation effects of 5 nM NAD+ on SIRT2 expression and deacetylase activity (n = 3), which is indicated by the level of α-tubulin AcK40. (B) NAD+ administration enhanced NER efficiencies in a SIRT2-dependent manner in neuronally differentiated 50B11 cells (n = 3). (C) Western blot showing the effects of administration of 5 nM NAD+ on SIRT1 expression and deacetylase activity in 50B11 cells in which SIRT1 expression is modified using siRNA against SIRT1 (n = 3). Deacetylase activity is indicated by acetylation of p53 at K373/K382 (AcK373.382). (D) SIRT1 expression and activity did not affect NAD+-mediated enhancement of NER efficiencies in 50B11 cells (n = 3). Statistical significance was assessed using 2-way ANOVA with Bonferroni’s correction (B and D). *P < 0.05; **P < 0.01; ***P < 0.001.