Figure 3. GFP–c-Rel transgene and c-Rel transgene overexpression limited to the GC stage and beyond phenocopy B cell–specific c-Rel overexpression.

(A) Representative flow cytometry plots of CD95^hiCD38^lo GCB cells and CD138⁺B220^lo plasma cells in GFP-Rel^TG CD19Cre^I/+ and control mice. Displayed numbers are median percentages (n ≥ 6). (B) CD95^hiCD38^lo GCB cell numbers and CD138⁺B220^lo plasma cell numbers. Individual data points obtained in 2 independent experiments are plotted. Bars and numbers below graphs are median values. *P ≤ 0.05, **P ≤ 0.01, unpaired t test. (C–F) Representative flow cytometry plots and cell numbers of CD95^hiCD38^lo GCB cells (C and E) and CD138⁺B220^lo plasma cells (D and F) in Rel^TG Cγ1Cre^I/+ and control mice. Displayed numbers in plots are median percentages (n ≥ 5). Individual data points obtained in 2 independent experiments are plotted in graphs. Bars and numbers below graphs are median values. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, unpaired t test. SPL, spleen; LN, lymph nodes; MLN, mesenteric lymph nodes; PP, Peyer’s patches. See Supplemental Figure 4F.