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. 2020 Apr 27;130(6):2845–2858. doi: 10.1172/JCI130597

Figure 6. pDC signaling capacity negatively correlates with plasma IFNα2 levels.

Figure 6

(A) p-IRF7 levels were measured in pDCs by flow cytometry after in vitro imiquimod stimulation of PBMCs obtained at the visit with the lowest IFNα response or from the only remaining sample available after ATI (ATI visit). Spearman’s correlations were determined between the MFI of p-IRF7 and the loss of IFNα-producing capacity, as measured by the fold decrease in pDCs producing IFNα in vitro at the ATI visit compared with levels detected at the preceding visit. (B and C) Plasma IFNα2 levels in RV397 participants were measured by SIMOA assay at the ATI visit. Spearman’s correlations were performed between the plasma IFNα2 levels at the ATI visit and the MFI of p-IRF7 in pDCs after in vitro imiquimod stimulation of PBMCs obtained at the ATI visit (B) or a pre-ATI visit (C). n = 8 participants from RV397 (all of whom received VRC01).