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. 2020 Apr 27;130(6):e132189. doi: 10.1172/JCI132189

Figure 6. Overexpression of activated YAP using PdgfrartTA in Mlck-mutant lungs partially rescues phenotypes.

Figure 6

(A–L) Representative images of stained sections from the alveolar regions of P8 lungs showing that Yap overexpression in Mlck-mutant lungs attenuated multiple phenotypes, including alveolar simplification (A–C), YAP staining intensity (D–F), α-SMA patterns (G–I), and p-MLC staining intensity (J–L). (M–O) Representative immunofluorescence staining for MLCK (red) in the alveolar region of P8 lungs showed a similar extent of MLCK inactivation in the Mlck-mutant lungs with or without Yap overexpression. (P) Quantification of alveolar simplification by MLI. *P = 0.0366, control versus PdgfrartTA tetO-Cre Mlckfl/fl tetO-YapS127A (n = 3); **P = 0.0035, PdgfrartTA tetO-Cre Mlckfl/fl versus PdgfrartTA tetO-Cre Mlckfl/fl tetO-YapS127A (n = 3); ***P = 0.0003, control versus PdgfrartTA tetO-Cre Mlckfl/fl (n = 3). Scale bars: 50 μm. Data represent the mean ± SEM. P values were determined by 1-way ANOVA with Tukey’s method to adjust for multiple comparisons.