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. 2020 May 4;130(6):2978–2991. doi: 10.1172/JCI132366

Figure 6. Marked accumulation of autophagosomes during late reperfusion is accompanied by upregulation of Rubicon.

Figure 6

(A) Three-month-old mice were subjected to 30 minutes of ischemia with reperfusion for the times indicated, and heart lysates were analyzed by Western blot using anti-Rubicon, anti-Vps34, anti-Atg14L, and anti–α-tubulin antibodies. (B) The ratio of Rubicon to α-tubulin was quantified (mean ± SEM, n = 4; *P < 0.05, **P < 0.01 versus Sham, 1-way ANOVA with Dunnett’s post hoc test). (C–F) Three-month-old mice were subjected to 30 minutes of ischemia and 24 hours of reperfusion. The mice were injected with 5 mg/kg Scrambled or Tat–Beclin 1 in the jugular vein 3 hours before ischemia (Pre), at the time of reperfusion (At rep.), or 3 hours after reperfusion (Post). (C) Schematic diagram of Tat–Beclin 1 injection during I/R. (D) Representative images of LV myocardial sections after Alcian blue and TTC staining (scale bar: 1 mm). Ratios of AAR to total LV (F) and infarction area to AAR (E) were compared (mean ± SEM, indicated n; *P < 0.05, **P < 0.01 vs. Scrambled, 1-way ANOVA with Dunnett’s post hoc test [E] and Tukey’s post hoc test [F]). See also Supplemental Figure 6.