Figure 5.

IL‐11 induced ADSCs proliferation, migration and anti‐apoptotic effects via IL‐11 alpha receptor. A and B, Representative images and quantitative analysis of ADSCs proliferation. EdU assay was performed 24 h after IL‐11 (20 ng/mL) or IL‐11 + anti‐IL‐11Rα (5 μg/mL) treatment. *P < .05 vs IL‐11. Two‐way ANOVA followed by Dunnett's post hoc test for statistical analyses; C and D, Representative images and quantitative analysis of ADSCs apoptosis. TUNEL staining assay was performed 24 h in ADSCs after IL‐11 (20 ng/mL) or IL‐11 + anti‐IL‐11Rα (5 μg/mL) treatment. H₂O₂: 200 μmol/L, 24 h. *P < .05 vs H₂O₂ + IL‐11. Two‐way ANOVA followed by Dunnett's post hoc test for statistical analyses; E and F, Cell migration analysis assessed by transwell assay 12 h in ADSCs with H₂O₂ after IL‐11 (20 ng/mL) or IL‐11 + anti‐IL‐11Rα (5 μg/mL) treatment. *P < .05 vs IL‐11. Two‐way ANOVA followed by Dunnett's post hoc test for statistical analyses; G and H, Western blots and quantification protein expression of P‐STAT3 in ADSCs after IL‐11 (20 ng/mL) or IL‐11 + anti‐IL‐11Rα (5 μg/mL) treatment. *P < .05 vs IL‐11. Unpaired Student's t test for statistical analyses; (n = 5 different fields, At least three independent experiments were performed)