Figure 6.

IL‐11 enhanced the efficacy of ADSCs therapy in a mouse model of hindlimb ischaemia. A and B, Expression of GFP fluorescence and the protein of flag in ADSCs after IL‐11 lentivirus transfection; C, representative blood perfusion images of mouse ischaemic hindlimbs after ADSCs transplantation; D, quantitative analysis of perfusion recovery by calculating the ischaemic/normal limb perfusion ratios. *P < .05 vs HI + MSC^con. n = 6. Two‐way ANOVA followed by Dunnett's post hoc test for statistical analyses; E, representative images of ADSCs in ischaemic hindlimbs 14 days after transplantation. Muscle tissue was immunostained for GFP (green), DAPI (blue). F, Quantitative analysis of GFP‐positive cells in the ischaemic limbs by calculating the number of GFP‐positive cells per field. n = 3. *P < .05 vs HI + MSC^con. Unpaired Student's t test for statistical analyses; G, representative images of Masson's trichrome staining of mouse ischaemic hindlimbs. Red represents viable muscle, and blue represents fibrosis