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. 2020 Apr 4;53(5):e12803. doi: 10.1111/cpr.12803

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© 2020 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Dental pulp stem cells (DPSCs) from sensory nerve‐deficient microenvironment display impaired properties. A, B, The IANx group displays lower rate of colony formation than Sham group. (**P < .01, n = 4). C, The CCK‐8 assay shows the poor proliferative capacity of IANx group. (***P < .005, n = 5). D, E, The cell apoptosis assay exhibits higher rate of apoptotic cells in IANx group. (**P < .01, n = 4). F‐H, The IANx group displays impaired osteogenesis capacity in mineralized nodules formation (F, G) and osteogenic related genes Alp, Runx2 and Opn (H). (*P < .05, ***P < .005, n = 6). I‐K, The IANx group shows impaired adipogenic potential in lipid droplets formation (I, J) and adipogenic‐related genes Lpl and Ppar‐γ (K). Scale bars: 50 μm. (*P < .05, **P < .01, ***P < .005, n = 6)