Skip to main content
. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Small. 2020 Jan 27;16(21):e1906674. doi: 10.1002/smll.201906674

Figure 4. Effects of bPEI-capped AgNPs and AgNCs on the biofilm formation of P. aeruginosa.

Figure 4.

Relative biofilm formation and bacterial viability of P. aeruginosa PAO1 in the presence of different concentrations of bPEI-capped AgNCs (A) and AgNPs (B). Fluorescence microscopy images of P. aeruginosa AH298-GFP incubated with 1 μM of AgNPs (C) and AgNCs (D) and untreated control that formed biofilms (E). A fluorescent strain of P. aeruginosa was used for fluorescence microscopy and ThT dye was used to stain the biofilms. Panels F, G and H represent the hyperspectral images (HSI) along with the SPR spectra of AgNPs, P. aeruginosa incubated with 1 μM AgNPs and P. aeruginosa control, respectively. Helium ion microscopy (HIM) and TEM micrographs of P. aeruginosa incubated with AgNPs (HIM: panel I, TEM: panel J), P. aeruginosa incubated with AgNCs (HIM: panel K, TEM: panel L) and P. aeruginosa control (HIM: panel M, TEM: panel N). ROS generation by P. aeruginosa, relative to positive control (H2O2), upon incubation with AgNPs and AgNCs (panel O).