Table 3.
Summary of kinetic measurements on the thio effect
| Nucleotide | kcat (s−1) | Km (μM) | kcat/Km (μM−1s−1) |
|---|---|---|---|
| dATP | 48 ± 2 | 17 ± 1 | 2.8 ± 0.2 |
| α-thio(Sp) dATP | 61 ± 4 | 35 ± 3 | 1.7 ± 0.2 |
Summary of kinetic measurements on the thio effect
| Nucleotide | kcat (s−1) | Km (μM) | kcat/Km (μM−1s−1) |
|---|---|---|---|
| dATP | 48 ± 2 | 17 ± 1 | 2.8 ± 0.2 |
| α-thio(Sp) dATP | 61 ± 4 | 35 ± 3 | 1.7 ± 0.2 |